How Location Dictates Biological Clocks of Species: Study in Beetles Offers New Insights

February.18.2021RESEARCH HIGHLIGHTS


Release Subtitle:
Scientists explore geographical variation in the biological clock of a new model organism, the Japanese red flour beetle

Release Summary Text:
Biological clocks are ubiquitous in living organisms and govern their behavioral pattern, from sleep-wake cycle to reproduction. Although they are well-understood, how they differ based on geographic location is unclear. In a new study, scientists from Japan report variations in the biological clocks of red flour beetles across the country, offering new insights into how they work.

Full text of release:
One of the most intriguing features in all living beings is the “biological clock”, an internal time-keeping mechanism that governs our behavioral pattern (such as the sleep-wake cycle). In fact, the biological clock dictates the developmental timing of various processes, such as when flowers bloom and insects reproduce. Biologists refer to these activities collectively as “circadian rhythms,” owing to the rhythmic pattern in which they occur.

Since their discovery, circadian rhythms have been studied extensively, and today we know a great deal about how they work. But, it is still not understood why, in some species, these rhythmic traits can vary based on geographical location (also called “cline”). While studies focusing on the relationship between the strength of “amplitude” of the rhythm (time variation in the level of a particular measure, such as activity level) and latitude (variation between north and south) have been conducted in fruit flies, the findings are contradictory.

In a new study published in PLoS One, scientists in Japan, led by Prof. Takahisa Miyatake from Okayama University, decided to explore the problem using a new biological model: the red flour beetle, a common insect found throughout Japan. He explains, “Organisms living in northern and southern countries should have different rhythm traits and it would be interesting to know why these differences exist. We came up with the idea that such geographical variation in traits could be easily studied in the red flour beetle, which can be collected in a wide range of latitudes from all over the country.”

Accordingly, the scientists collected more than 35 places of the red flour beetles from 37 rice mills in different parts of Japan and nurtured them under laboratory conditions emulating their natural environment. After an initial nurturing period of 20 days, they assessed the circadian rhythm of individual beetles (more than 1500 samples) by monitoring their locomotor activity (movement) for 10 days.

The findings showed that the rhythm strength decreased with increasing latitude such that beetles from the northern parts showed weaker circadian rhythms than their southern counterparts. However, changing longitudes (east to west) showed no significant difference. Interestingly enough, despite the large sample size, they did not observe any variation in the rhythm period with latitude or longitude, which contradicted the findings of previous studies.

The scientists ascribed the weaker rhythmic amplitude at higher altitudes to its more extreme and harsh environments, which possibly had more influence on the beetle’s behavior than its biological clock. As for the unchanging rhythm period with location, they speculated that the flow of genetic material from one beetle population to another was likely more than hypothesized, thus washing out their genetic differences and leading to uniform rhythm periods.

Prof. Miyatake is excited by these findings and their possible implications. He concludes, “Many insects are expanding their distribution areas to the north due to global warming, and the nature of their circadian rhythms may change accordingly. We may be able to open up a new research field to see if the nature of variation in the biological clock of insects is also applicable to humans!”

Does this mean our bodies might be working in a certain way depending on our location? We don’t entirely know yet, but hopefully, future studies will reveal a lot more.

Release URL: https://www.eurekalert.org/pub_releases/2021-02/ou-hld021821.php

Reference:
Title of original paper: Amplitude of circadian rhythms becomes weaken in the north, but there is no cline in the period of rhythm in a beetle
Journal: PLoS One
DOI: http://dx.doi.org/10.1371/journal.pone.0245115

Contact Person: MIYATAKE Takahisa
Dr. Takahisa Miyatake is a Professor at the Graduate School of Environmental and Life Science at Okayama University, Japan. His research areas include evolutionary ecology, insect behavior, applied entomology, and ethology. As a senior researcher and professor, he has more than 200 publications to his credit with over 4,000 citations. He also received the Hidaka Prize from Japan Ethological Society in 2016.


Variations in biological clocks of red flour beetles based on location. Sampling points of the beetle (upper left), act graph machine to measure rhythm (upper right), adults of the red flour beetle (central), latitude and activity amplitude (lower left) and longitude and activity amplitude (lower left).
Credit:2021 Okayama University

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